FastQCFastQC Report
Tue 4 May 2021
HPSI1113i-qorq_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHPSI1113i-qorq_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46419838
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG40350.015.5669253
CCCGTAT51200.015.5063051
GTATCGA49000.012.4653594
CCGTATC61800.011.6161952
ATCGCGC47950.011.15353667
TATCGAA60750.010.9044515
ATCGAAG93850.09.8141086
CTCCGAT73150.09.720831
TCGACCG37700.09.70081431
CGACCGT37750.09.59624932
CTCGTTT82400.09.1322911
GTCCGTT35200.09.021851
CCGTAAT37750.08.9610641
CGTGTTA60900.08.89126259
CATCGCG72100.08.5655866
CTCGTAT43550.08.3224581
CGACCTA22200.08.2385444
CGCGAGT32350.08.10632913
CTCGGAT122300.08.0157161
TACGCAC38850.07.993239451
Produced by FastQC (version 0.11.5)